The Anti-Cancer Synergistic Effect Of Colchicine On Cancer Cells Proliferation (In Vitro Study) And Chloroquine
Main Article Content
Abstract
This study aimed to investigate the possible in vitro cytotoxicity of Colchicine and Chloroquine. The powder was drugs from Colchicines, Chloroquine, and combination drugs. evaluated by MTT assay for cytotoxicity against AMJ13, breast cancer cell lines. The results showed synergistic cytotoxicity against breast cancer cells by Colchicine, Chloroquine and combination drugs has potent cytotoxicity against AMJ13 cells where IC50 of Colchicine treated range( 2.351 to 20.73 was 6.982) for AMJ13, IC50 of Chloroquine treated range (2.696 to 10.9 was 5.42) for AMJ13, IC50 of combination drugs used CompuSyn Isobologram when it was for AMJ13. The cytotoxicity of drugs with Colchicine, Chloroquine and combination drugs showed a synergistic activity on AMJ13 cell lines). Colchicine, Chloroquine and combination drugs have potent cytotoxicity against AMJ13 cell lines.
Article Details
This work is licensed under a Creative Commons Attribution 4.0 International License.
References
Ahmed SE, Sarhat ER, Awni N, et al (2021) Altered Serum Marker of Adipokines Profile in Breast Cancer Women. Indian J Forensic Med Toxicol 15:2599
Hamad MS, Sarhat ER, Khalaf SJ, et al (2020) Characteristic Abnormalities In Serum Biochemistry In Patients With Breast Cancer. Syst Rev Pharm 11:1967–1971
Sarhat ER, Abid IM, Kamel NA, et al (2021) Changes of serum Interleukin and Chemerin levels in patients with Polycystic Ovary syndrome. J Adv Pharm Educ Res Oct-Dec 11:
Bray F, Ferlay J, Soerjomataram I, et al GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. Glob cancer Stat 2018
Berrino F, Lutz JM, De Angelis R, et al Cancer survival in five continents: a worldwide population-based study (CONCORD)
Al-Hashimi MMY, Wang XJ (2014) Breast cancer in Iraq, incidence trends from 2000-2009. Asian Pacific J cancer Prev 15:281–286
Mualla FHF, Al-Alwan NAS (2014) Promoting clinical breast examination as a screening tool for breast cancer in Iraq. Iraqi Natl J Nurs Spec 27:
Pandrangi SL, Raju Bagadi SA, Sinha NK, et al (2014) Establishment and characterization of two primary breast cancer cell lines from young Indian breast cancer patients: mutation analysis. Cancer Cell Int 14:1–20
Liu L, Yan L, Liao N, et al (2020) A review of ULK1-mediated autophagy in drug resistance of cancer. Cancers (Basel) 12:352
Bhattacharya S, Das A, Datta S, et al (2016) Colchicine induces autophagy and senescence in lung cancer cells at clinically admissible concentration: potential use of colchicine in combination with autophagy inhibitor in cancer therapy. Tumor Biol 37:10653–10664
Wang X, Zhang H, Chen X (2019) Drug resistance and combating drug resistance in cancer. Cancer Drug Resist 2:141
Yu Q, Liu L, Wang P, et al (2017) EMAP-II sensitize U87MG and glioma stem-like cells to temozolomide via induction of autophagy-mediated cell death and G2/M arrest. Cell Cycle 16:1085–1092
Al-Shammari AM, Salman MI, Saihood YD, et al (2016) In vitro synergistic enhancement of Newcastle Disease Virus to 5-fluorouracil cytotoxicity against tumor cells. Biomedicines 4:3
Al-Shammari AM, Jalill RDA, Hussein MF (2020) Combined therapy of oncolytic Newcastle disease virus and rhizomes extract of Rheum ribes enhances cancer virotherapy in vitro and in vivo. Mol Biol Rep 47:1691–1702
Al-Ziaydi AG, Al-Shammari AM, Hamzah MI, et al (2020) Newcastle disease virus suppress glycolysis pathway and induce breast cancer cells death. Virusdisease 31:341–348
Chou T-C (2010) Drug Combination Studies and Their Synergy Quantification Using the Chou-Talalay MethodSynergy Quantification Method. Cancer Res 70:440–446
Al-Shammari AM, Allak WJK, Umran M, et al (2015) Angiogenesis factors associated with new breast cancer cell line AMJ13 cultured in vitro. Adv Breast Cancer Res 4:100
Freshney RI (2015) Culture of animal cells: a manual of basic technique and specialized applications. John Wiley & Sons
Katiyar RS, Singhvi NR, Kushwaha R V, et al (2009) VA-mycorrhizal association in arjuna and jamun trees in forest of Bhandara region, Maharashtra, India. Int J Plant Sci 4:229–232
Mantena SK, Sharma SD, Katiyar SK (2006) Berberine, a natural product, induces G1-phase cell cycle arrest and caspase-3-dependent apoptosis in human prostate carcinoma cells. Mol Cancer Ther 5:296–308
Lazarus DB, Kotrc B, Wulf G, Schmidt DN (2009) Radiolarians decreased silicification as an evolutionary response to reduced Cenozoic ocean silica availability. Proc Natl Acad Sci 106:9333–9338
Al-Shammari AM, Abdullah AH, Allami ZM, Yaseen NY (2019) 2-Deoxyglucose and Newcastle disease virus synergize to kill breast cancer cells by inhibition of glycolysis pathway through glyceraldehyde3-phosphate downregulation. Front Mol Biosci 6:90
Arora R, Schmitt D, Karanam B, et al (2015) Inhibition of the Warburg effect with a natural compound reveals a novel measurement for determining the metastatic potential of breast cancers. Oncotarget 6:662
Foucquier J, Guedj M (2015) Analysis of drug combinations: current methodological landscape. Pharmacol Res Perspect 3:e00149
Rodea-Palomares I, Petre AL, Boltes K, et al (2010) Application of the combination index (CI)-isobologram equation to study the toxicological interactions of lipid regulators in two aquatic bioluminescent organisms. water Res 44:427–438
W. Humphrey R, M. Brockway-Lunardi L, T. Bonk D, et al (2011) Opportunities and challenges in the development of experimental drug combinations for cancer. J Natl Cancer Inst 103:1222–1226
Guo X, Zhang X, Wang T, et al (2016) 3-Bromopyruvate and sodium citrate induce apoptosis in human gastric cancer cell line MGC-803 by inhibiting glycolysis and promoting mitochondria-regulated apoptosis pathway. Biochem Biophys Res Commun 475:37–43